Core Particles of Hepatitis B Virus and Ground Squirrel Hepatitis Virus I. Relationship Between Hepatitis B Core Antigen- and Ground Squirrel Hepatitis Core Antigen-Associated Polypeptides by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Peptide Mapping
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چکیده
منابع مشابه
Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli
DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...
متن کاملImmunogenicity of peptide fusions to hepatitis B virus core antigen.
Several gene fusions have been constructed in which coding sequences for antigenic regions of the pre-S sequences of hepatitis B virus, hepatitis B surface antigen, and the envelope protein of human immunodeficiency virus were linked to the 3' end of that for the first 144 residues of hepatitis B core antigen. The sequences were expressed efficiently in Escherichia coli to give stable products ...
متن کاملTree squirrel hepatitis B virus: antigenic and structural characterization.
Tree squirrel hepatitis B virus (THBV)-associated particles isolated from the livers of naturally infected animals share one or more antigenic determinants with hepatitis B surface antigen in solid-phase immunoassays. Characterization of THBV-associated polypeptides by sodium dodecyl sulfate/polyacrylamide gel electrophoresis reproducibly demonstrated major components with apparent sizes of 15....
متن کاملHepatitis B surface antigen polypeptides: artifactual bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis caused by aggregation.
Hepatitis B surface antigen, subtype ad, was purified and studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. Two major bands with molecular weights of 23,500 and 27,500 and several weaker bands with higher molecular weights were observed. When the low-molecular-weight bands and the group of high-molecular-weight bands were excised from the gel, elute...
متن کاملSignificance of Hepatitis B Core Antibody as the Only Marker of Hepatitis B Virus Infection in High Risk Patients
Background and Objective: Presence of hepatitis B core antibody (anti-HBc) in the absence of hepatitis B surface antigen (HBsAg) and hepatitis B surface antibody (anti-HBs) is defined, as isolated anti-HBc. little is known about the clinical significance of the isolated anti-HBc in hepatitis B virus (HBV) infections. The aim of this study was to assess the significance of anti-HBc as the onl...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1982
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.43.2.687-696.1982